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2 edition of Genetic and physical studies of a Streptomyces coelicolor plasmid. found in the catalog.

Genetic and physical studies of a Streptomyces coelicolor plasmid.

Mervyn James Bibb

Genetic and physical studies of a Streptomyces coelicolor plasmid.

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Published by University of East Anglia in Norwich .
Written in English


Edition Notes

Thesis (Ph.D.) - University of East Anglia, School of Biological Sciences, 1978.

ID Numbers
Open LibraryOL13845775M

  Apples. Although 7 E. coli OH7 outbreaks associated with apple juice or cider occurred between and , there have been no reports of foodborne illnesses linked to consumption of fresh apples (Rangel and others ).The presence of pathogens on the surface of apples has implications for safety of raw material for the fresh juice and fresh‐cut fruit by: This banner text can have markup.. web; books; video; audio; software; images; Toggle navigation. Doubly ionized carbon is an important, and many times the major, ionization state of carbon in planetary nebulae and HII regions. In this poster we report the results of imagery of five PNe (NGC , NGC , NGC , NGC , and NGC ) with the HST WFPC2 using the FW filter, which includes emission from the C III]λλ, line.   In Streptomyces coelicolor, CCR of genes that are involved in the use of secondary carbon sources seems to be independent of the PTS Instead, glucose kinase is Cited by:

The present invention relates to a shuttle BAC vector for facilitating the cloning, transfer and heterologous expression of a biosynthetic gene cluster of streptomyces secondary metabolites. The present invention also used this vector to enhance the process of cloning secondary metabolite biosynthetic gene clusters from streptomyces without advanced generation and screening of cosmid or BAC Author: リュー ホングボ, ヒー ミン.


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Paper

Paper

Genetic and physical studies of a Streptomyces coelicolor plasmid. by Mervyn James Bibb Download PDF EPUB FB2

This chapter on Streptomyces focuses on the need to draw attention to the progress in developing systems for genetic manipulation in actinomycetes. A significant fraction of this progress has depended on approaches first used in Streptomyces species.

Interestingly, the largest open reading frame in IS is related to that of members of the IS family. Education. Bibb was educated at the University of East Anglia where he graduated with a BSc in Biological Sciences and was awarded a PhD in for studies of plasmids in Streptomyces coelicolor.

Research. Bibb does research on. The regulation of secondary metabolism (antibiotic production) in streptomycetes; Functional genomics in Streptomyces, particularly transcriptome and proteome analysisAlma mater: University of East Anglia. Abstract. Genetic exchange in Streptomyces was first revealed when prototrophic recombinants were recovered from mixed cultures of pairs of auxotrophic derivatives of several wild-type strains (1, 8, 25, 63, 65).

A conjugative mechanism, rather than transformation or transduction, was invoked to account for gene exchange because no recombinants were detected without prolonged physical contact Cited by: The complete nucleotide sequence including the novel telomere sequence of Streptomyces linear plasmid pSHK1 consists of ,bp, genes, in which 51 genes resemble those of the linear.

Genetic Instability in Streptomycetes. Authors; Authors and affiliations Bibb, M.J., and Hopwood, D.A.,Genetic studies of the fertility plasmid SCP2 and its SCP2⋆ variants in Genetic control of fertility in Streptomyces coelicolor A3(2) plasmid involvement in the interconversion of UF strains, J.

Gen. Microbiol. Cited by: 1. Genetic mapping of the chromosome of Streptomyces coelicolor A3(2) began with the analysis of haploid recombinants selected from matings. A physical map of the S. coelicolor chromosome has recently been constructed, using pulsed-field gel electrophoresis of AseI and DraI fragments.

The genetic map was found to be reasonably congruent with the physical map over the well-marked by: 2. Maniatis' book. For those totally unfamiliar with streptomyces, the standards cloning organism is S lividians although the most comprehensive genetic map is of S coelicolor.

There are a variety of high, medium and low copy number plasmid vectors available for streptomyces and also phage vectors based on Cited by: 1.

Plasmid-mediated conjugation (sometimes without identification of the plasmid involved) has been used widely for the construction of genetic maps of Streptomyces strains.

27 In S. coelicolor A3(2), two plasmids have been implicated: SCP2, a kb ccc plasmid (and its SCP2* variants with enhanced transfer ability7,21), and SCP1, a linear plasmid Cited by: Streptomyces lavendulae is a species of bacteria from the genus Streptomyces.

It is isolated from soils globally and is known for its production of medically useful biologically active metabolites. Carbon utilization studies on S.

lavendulae have shown good or moderate growth with chromosomes are linear and fairly large, Mb. In Class: Actinobacteria.

coelicolor contains 11 chromosomal and three plasmid-located wbl genes, among which five are widely conserved among actinomycetes, whereas the rest are genus- or species-specific S.

coelicolor. Streptomyces is a genus of Gram-positive bacteria that grows in various environments, and its shape resembles filamentous fungi. The morphological differentiation of Streptomyces involves the formation of a layer of hyphae that can differentiate into a chain of spores.

The most interesting property of Streptomyces is the ability to produce bioactive secondary metabolites, such as antifungals Cited by:   Recombination was also of importance in the mapping of production genes.

The model for such investigations was the genetic map of Streptomyces coelicolor 3 which was found to be very similar to those of other Streptomyces species, such as Streptomyces bikiniensis, Streptomyces olivaceous, Streptomyces glaucescens and Streptomyces rimosus.

1. Introduction. Classic genetic studies are based on correlating genetic alterations with the resulting phenotypes. Several important signaling pathways, including mTOR [1,2], apoptosis [], autophagy [] and Hippo [5,6] pathways, have been discovered by classic the fact that some lethality-causing genes or essential genes are impossible to overexpress or knock out, fine Cited by: 1.

A bacterial strain, Streptomyces albogriseolus LBX-2, was isolated from a soil sample in Chengdu, China. albogriseolus LBX-2 is an aerobic and Gram-positive microorganism that is capable of using the polyethylene as the sole carbon source. Results of scanning electron microscopy and tensile tests indicated that S.

albogriseolus LBX-2 could cause the damages to polyethylene (PE).Cited by: 2. Abstract The 8–9-Mb Streptomyces chromosome is linear, with a “core” containing essential genes and “arms” carrying conditionally adaptive genes that can sustain large deletions in the laboratory.

Bidirectional chromosome replication from a central oriC is completed by “end-patching,” primed from terminal proteins covalently bound to the free 5′-ends. Plasmid-mediated. The classic comprehensive and authoritative textbook on bacterial molecular genetics.

Features completely revised and updated material and new chapters, incorporating the most recent advances in the field since publication of the third edition in Provides thought questions, problems, and suggested reading lists for each chapter that test.

In the first example of stable cointegrate formation in Streptomyces, the SCP1 plasmid was inserted into the S. coelicolor chromosome to yield a donor with fertility properties designated NF.

Recombinants arose from matings between NF and SCP1 − strains with frequencies approaching the % transfer shown by the free by: Resistance of Streptomyces isolate against Basta (Bialophos) herbicide was tested on three doses of Basta (2, 4 and 6 g/L) in starch nitrate agar medium (SNAM). The stressed cultures were studied to find gene confers resistance for herbicide.

The bar gene was isolated via polymerase chain reaction (PCR) from the culture. This local isolate was identified as Streptomyces coelicolor : A.

Altalhi, M. Sonya, A. Sadik. From the beginning of his research career he has pioneered studies of the genetics of Streptomyces, a member of the group of filamentous, Gram-positive soil bacteria called the actinomycetes that are preeminent producers of polyketide and other antibiotics.

Over the last 10 years or so he has harnessed this genetic system to help to illuminate. Traditional three-domain fungal and bacterial laccases have been extensively studied for their significance in various biotechnological applications.

Growing molecular evidence points to a wide occurrence of more recently recognized two-domain laccase-like multicopper oxidase (LMCO) genes in Streptomyces spp.

However, the current knowledge about their ecological role and distribution in Cited by: We report the identification of the biosynthetic gene cluster for the unusual antibiotic anthracimycin (atc) from the marine derived producer strain Streptomyces sp. T isolated off St.

John’s Island, Singapore. The 53 bps atc locus includes a trans-acyltransferase (trans-AT) polyketide synthase (PKS), and heterologous expression in Streptomyces coelicolor resulted in anthracimycin.

The prize consists of a $ book award, a framed certificate, and a copy of Dr. Henderson’s book, “Fitness of the Environment.” Each year a committee of the Board of Tutors in Biochemical Sciences reviews all theses that have been evaluated as outstanding (A) by both thesis readers.

Wbl proteins contain a [4Fe–4S] cluster, were first identified in Streptomyces coelicolor, and are widely distributed in actinobacteria (2,3). Seven Wbl proteins are found in M. tuberculosis, and they play versatile roles in diverse biological processes including redox homeostasis, cell division, nutrition starvation and antibiotic Author: T.

Wan, S. Li, D.G. Beltran, A. Schacht, L. Zhang, D.F. Becker. Streptomyces scientists at the John Innes Centre oversaw the genome sequencing of Streptomyces coelicolor A3 (2) by the Sanger Centre in Cambridge, and the subsequent development of a range of genomic tools by UK consortia.

Enter search terms. Keep search filters New search. Advanced search. Actinobacteria are Gram-positive bacteria with high G+C DNA content that constitute one of the largest bacterial phyla, and they are ubiquitously distributed in both aquatic and terrestrial ecosystems.

Many Actinobacteria have a mycelial lifestyle and undergo complex morphological differentiation. They also have an extensive secondary metabolism and produce about two-thirds of all Cited by: A comprehensive collection of readily reproducible techniques for the manipulation of recombinant plasmids using the bacterial host E.

coli. The authors describe proven methods for cloning DNA into plasmid vectors, transforming plasmids into E. coli, and analyzing recombinant clones.

The present invention relates to material derived from the SCP1 plasmid of Streptomyces coelicolor A3(2) and methods and uses relating thereto, in particular to material derived from the gene cluster for methylenomycin A biosynthesis. Underlying the invention is work carried out by the inventors in sequencing and deducing the function of various genes in the methylenomycin A biosynthetic gene.

Thioviridamide is a structurally novel ribosomally synthesized and post-translational modified peptide (RiPP) produced by Streptomyces olivoviridis NA It is characterized by a structure that features a series of thioamide groups and possesses potent antiproliferative activity in cancer cell lines.

Its unusual structure allied to its promise as an anticancer compound led us to Cited by: Classic genetic studies are based on correlating genetic alterations with the resulting phenotypes. Several important signaling pathways, including mTOR [1,2], apoptosis [], autophagy [] and Hippo [5,6] pathways, have been discovered by classic the fact that some lethality-causing genes or essential genes are impossible to overexpress or knock out, fine-tuning their expression Cited by: 1.

Streptomyces coelicolor differs from Caulobacter and Bacillus in that it grows as filaments called hyphae. A member of the actinomycetes, from which many important antibiotics are produced, S. coelicolor is a favorite model organism for studies of both secondary metabolism and by: The giant linear plasmid SCP1 can integrate into the central region of the linear chromosome of Streptomyces coelicolor A3(2).

Nucleotide sequence analysis around the target site for SCP1 integration in strain M identified a total of five copies of four insertion sequences (ISs) in a kb DNA stretch.

Three of the four (IS, IS, and IS) are new IS elements, and the other is ISCited by: 8. The jaspamide/chondramide family of depsipeptides are mixed PKS/NRPS natural products isolated from marine sponges and a terrestrial myxobacterium that potently affect the function of the actin cytoskeleton.

As a first step to improve production in heterologous host cells and permit genetic approaches to novel analogs, we have cloned and characterized the chondramide biosynthetic genes from Cited by: (57) [Summary] The present invention relates to an isolated transducing phage, a method for isolating a transducing phage, and a method using a transducing phage, for example, transferring at least one nucleic acid fragment from a donor microorganism to a recipient microorganism, and Producing secondary metabolites.

The transducing phage has a broad host range, and is a microorganism. SUMMARY Tuberculosis (TB), one of the oldest known human diseases. is still is one of the major causes of mortality, since two million people die each year from this malady.

TB has many manifestations, affecting bone, the central nervous system, and many other organ systems, but it is primarily a pulmonary disease that is initiated by the deposition of Mycobacterium tuberculosis, Cited by: Bacteria (/ b æ k ˈ t ɪər i ə / (); common noun bacteria, singular bacterium) are a type of biological constitute a large domain of prokaryotic lly a few micrometres in length, bacteria have a number of shapes, ranging from spheres to rods and ia were among the first life forms to appear on Earth, and are present in most of its : Bacteria, Woese, Kandler & Wheelis, Quantitative relationships for speci?c growth rates and macromolecular compositions of Mycobacterium tuberculosis, Streptomyces coelicolor A3(2) and Escherichia coli B/r: an integrative theoretical approach.

Microbiology,pp Farr, S.B. and Kogama, T. ().Oxidative Stress Responses in Escherichia coli and Salmonella. Unfortunately, this book can't be printed from the OpenBook.

If you need to print pages from this book, we recommend downloading it as a PDF. Visit to get more information about this book, to buy it in print, or to download it as a free PDF. Lawrence J. Henderson Prize The Henderson Prize honors the work of Professor Henderson (), who began teaching at Harvard in and is best known for his research on blood biochemistry.

The prize consists of a $ book award, a framed certificate, and a copy of Dr. Henderson's book, "Fitness of the Environment." Each year a committee of the Board of Tutors in Biochemical Sciences. The role of DNA methylation in silencing. DNA methylation is an important epigenetic mark involved in cell differentiation and organ and tissue development, which plays a crucial role in the establishment of genomic imprinting (parent-dependent silencing of alternative alleles) in both male and female germ by: 6.

High diversity and a rapid succession of Streptomyces two-domain LMCO genes were found in the six representative samples with 8 distinct clades of sequence types, among which most sequences clustered with the reference two-domain LMCO genes of Streptomyces coelicolor, Streptomyces violaceusniger, Streptomyces griseus, Streptomyces ipomoeae, and.Rediscovery of Mendel's paper.

Three independent researchers (Austrian agricultural botanist Erich Tschermak, German botanist Karl Correns, and Dutch botanist Hugo de Vries) published responses to an paper by Gregor Mendel titled ‘Versuche über Pflanzen-Hybriden’ (Experiments on Plant Hybridisation) which gave the results of eight years of crossing experiments with 22 true-breeding.As a result of the molecular genetic analysis of development similar mechanisms for the regulation of gene expression are found in a wide range of organisms.

In "Development - the Molecular Genetic Approach" these common mechanisms as well as the specific events leading to a differentiated cell are described. Particular items treated are, for example, how asymmetry is achieved, how.